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pfu x core kit  (Jena Bioscience)


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  • 90

    Structured Review

    Jena Bioscience pfu x core kit
    Pfu X Core Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+x+core+kit/Pfu-X+Core+Kit/pmc09245083-62-19-22
    Average 90 stars, based on 1 article reviews
    pfu x core kit - by Bioz Stars, 2026-09
    90/100 stars

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    Cloning:

    Article Title: Genome-wide expression and methylation analyses reveal aberrant cell adhesion signaling in tyrosine kinase inhibitor-resistant CML cells
    Article Snippet: After addition of 200 μl PBS, the plates were measured using 494 nm as absorbance and 517 nm as emission wavelength at an Infinite M200 Pro device (Tecan Group, Ltd.). .. FN1 coding plasmids (Gene ID: 2335, ABIN3996197, antibodies-online) were subcloned into the pSelect–puromycin-mcs vector (Sigma-Aldrich; Merck KGaA) using the Pfu-X Core kit (Jena Bioscience) using the following set of primers: FN1_pSELECT_forward, 5′-GCGTGTCGACGGATCATGCTTAGGGGTCCGGGG-3′ and reverse, 5′-GCCAGCTAGCCCATGTTACTCTCGGGAATCTTCTCTGTC-3′ with annealing at 61°C, the restriction enzymes BamHI and NcoI (New England BioLabs, Inc.), cloning enhancer and the In-Fusion HD kit (Takara Bio Europe SAS). .. Cells (2×10 6 ) were transfected with 5 μg of the respective plasmid (pSELECT-empty; pSELECT-FN1) using nucleofection and the nucleofector 2 b device (Lonza Group Ltd.).



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    90
    Jena Bioscience pfu x core kit
    Pfu X Core Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+x+core+kit/Pfu-X+Core+Kit/pmc09245083-62-19-22
    Average 90 stars, based on 1 article reviews
    pfu x core kit - by Bioz Stars, 2026-09
    90/100 stars
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